Anti-Tyrosinase Antibody [SPM360] (A250240)

Anti-Tyrosinase Antibody [SPM360]

Mouse monoclonal [SPM360] antibody to Tyrosinase.

This antibody recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.

Flow Cytometry, IF, IHC-P

Flow Cytometry: 0.5-1 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human

Recombinant Tyrosinase protein.

Mouse

Monoclonal

SPM360

IgG2a

kappa

Protein A/G chromatography.

200 µg/ml

70-80 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Tyrosinase Antibody [SPM360] - BSA and Azide free (A253420).

ATN, CMM8, LB24 AB, LB24-AB, Monophenol monooxygenase, OCA1, OCA1A, OCAIA, Oculocutaneous albinism IA, SHEP3, SK29 AB, SK29-AB, Tumor rejection antigen AB, TYR, tyrosinase (oculocutaneous albinism IA), TYRO_HUMAN