Anti-Tuberin Antibody (A8414)

Name: Anti-Tuberin Antibody
Brand: Antibodies.com
SKU: A8414
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Tuberin for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Tuberin Antibody

Description

Rabbit polyclonal antibody to Tuberin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1160-1255 of human TSC2 (NP_000539.2).

Sequence

TAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

200 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FLJ43106, LAM, OTTHUMP00000158940, OTTHUMP00000198394, OTTHUMP00000198395, PPP1R160, Protein phosphatase 1, regulatory subunit 160, TSC complex subunit 2, tsc2, TSC2_HUMAN, TSC4, TSC4 gene, formerly, TSC4, formerly, Tuberous sclerosis 2, Tuberous sclerosis 2 protein, Tuberous sclerosis 2 protein homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Tuberin Antibody (A8414)

Western blot analysis of extracts of SH-SY5Y cells, using Anti-Tuberin Antibody (A8414) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-Tuberin Antibody (A8414)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-Tuberin Antibody (A8414) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Tuberin Antibody (A8414)

Immunofluorescence analysis of BALB-3T3 cells using Anti-Tuberin Antibody (A8414) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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