TrkA (phospho Tyr680 + Tyr681) Cell Based ELISA Kit (A102464)

TrkA (phospho Tyr680 + Tyr681) Cell Based ELISA Kit

Cell-based (qualitative)

This TrkA (phospho Tyr680 + Tyr681) Cell Based ELISA Kit allows for the detection of TrkA (phospho Tyr680 + Tyr681) and the effects that certain stimulation conditions have on TrkA (phospho Tyr680 + Tyr681) expression in different cell lines. Qualitative determination of TrkA (phospho Tyr680 + Tyr681) concentration is achieved by an indirect ELISA format. In essence, the TrkA (phospho Tyr680 + Tyr681) is captured by Anti-TrkA (phospho Tyr680 + Tyr681) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this TrkA (phospho Tyr680 + Tyr681) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-TrkA Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Colorimetric

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 6 months.

High affinity nerve growth factor receptor, MTC, Neurotrophic tyrosine kinase receptor type 1, NTRK1, TRK, Trk-A, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A