Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
RING finger protein 87, RNF 87, RNF87, TRIM4_HUMAN, Tripartite motif containing 4, Tripartite motif protein 4, Tripartite motif protein TRIM4, Tripartite motif-containing protein 4
Figure 1: Western Blot - Anti-TRIM4 Antibody (A90582)
Western blot analysis of extracts of various cell lines, using Anti-TRIM4 Antibody (A90582) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-TRIM4 Antibody (A90582) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-TRIM4 Antibody (A90582) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-TRIM4 Antibody (A90582) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
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