Anti-TRIM29 Antibody (A84758)

TRIM29 expression in Peripheral Blood Mononucleocyte (A) and HeLa (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-TRIM29 Antibody (A84758) at 1µg/ml (A) or 0.1 µg/ml (B) and detected by chemiluminescence.

TRIM29 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-TRIM29 Antibody (A84758) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Cytoplasmic and intermediate filament staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

TRIM29 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-TRIM29 Antibody (A84758) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

TRIM29 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-TRIM29 Antibody (A84758) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.