Anti-TRIM2 Antibody (A83321)

Name: Anti-TRIM2 Antibody
Brand: Antibodies.com
SKU: A83321
Price: 480.0 USD
Availability: InStock

This product is identical to Abcam (ab3942). Discover more for less.

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$480

Goat polyclonal antibody to TRIM2 for ELISA, WB, IHC and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-TRIM2 Antibody

Description

Goat polyclonal antibody to TRIM2.

Applications

ELISA, WB, IHC, Flow Cytometry

Dilutions

ELISA: 1:16,000, WB: 0.5-1µg/ml, IHC: 6-12µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human, Rat

Cross Reactivity

This antibody cross reacts with mouse TRIM3.

Immunogen

Synthetic peptide corresponding to the C terminal region of human TRIM2.

Sequence

DSGNHCFKVYRYLQ

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

75-85 kDa

Predicted MW

83.3kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

E3 ubiquitin protein ligase TRIM2, KIAA0517, RING finger protein 86, RNF86, TRIM2_HUMAN, Tripartite motif containing 2, Tripartite motif protein 2, Tripartite motif protein TRIM2, Tripartite motif-containing 2, Tripartite motif-containing protein 2

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-TRIM2 Antibody (A83321)

Anti-TRIM2 Antibody (A83321) (0.5µg/ml) staining of Rat Brain (A) + Peptide (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-TRIM2 Antibody (A83321)

Anti-TRIM2 Antibody (A83321) (6.3µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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Figure 3: Flow Cytometry - Anti-TRIM2 Antibody (A83321)

Anti-TRIM2 Antibody (A83321) - Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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