Recombinant human monoclonal [PY159] antibody to TREM1.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human
Host
Human
Clonality
Monoclonal
Clone ID
PY159
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 143.66 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Anti-TREM1 Antibody [PY159] - Low endotoxin, Azide free (A324278) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
Human TREM1 HEK293 cells were stained with Anti-TREM1 Antibody [PY159] - Low endotoxin, Azide free (A324278) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 0.7744nM.
The endocytosis ratio of Anti-TREM1 Antibody [PY159] - Low endotoxin, Azide free (A324278) by hu-TREM1-HEK293 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 80% at antibody concentration of 0.3 nM.
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