Anti-TRAP1 Antibody [3H4-2H6] (A305019)

Name: Anti-TRAP1 Antibody [3H4-2H6]
Brand: Antibodies.com
SKU: A305019
Price: 490.0 USD
Availability: InStock

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$490

Mouse monoclonal [3H4-2H6] antibody to TRAP1 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-TRAP1 Antibody [3H4-2H6]

Description

Mouse monoclonal [3H4-2H6] antibody to TRAP1.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Purified recombinant Human TRAP2.

Host

Mouse

Clonality

Monoclonal

Clone ID

3H4-2H6

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~75 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Heat shock protein 75 kDa, Heat shock protein 75 kDa, mitochondrial, HSP 75, HSP75, HSP90L, mitochondrial, TNF receptor associated protein 1, TNFR-associated protein 1, TRAP-1, TRAP1_HUMAN, Tumor necrosis factor type 1 receptor-associated protein

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-TRAP1 Antibody [3H4-2H6] (A305019)

Western blot analysis of Human, rat human A431 and rat brain membrane cell lysates showing detection of ~75 kDa Trap1 protein using Anti-TRAP1 Antibody [3H4-2H6] (A305019) at 1:1,000 for 1 hour at room temperature. Lane 1: MW ladder. Lane 2: human lysate, A431. Lane 3: rat lysate, rat brain membrane (RBM). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was HRP Goat Anti-Rabbit at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 15 min at room temperature. Predicted/Observed Size: ~75 kDa.

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Figure 2: Immunofluorescence - Anti-TRAP1 Antibody [3H4-2H6] (A305019)

Immunocytochemistry/Immunofluorescence analysis of mouse myoblast cell line C2C12 , fixed in 4% formaldehyde for 15 min at room temperature, using Anti-TRAP1 Antibody [3H4-2H6] (A305019), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:5,000 for 5 minutes room temperature. Localization: Nucleus. Magnification: 60X.

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