Anti-Transferrin Receptor Antibody [OX-26] (A281425)

Anti-Transferrin Receptor Antibody [OX-26]

Mouse monoclonal [OX-26] antibody to Transferrin Receptor.

This antibody recognises rat CD71, also known as transferrin receptor, a homodimeric type II transmembrane protein, expressed by all proliferating cells and cells with a requirement for iron, including reticulocytes and capillary endothelium in brain. Clone 0X-26 also binds to a number of non-dividing normal tissues.The balance between a sufficient amount of iron uptake and prevention of accumulation of excess iron within a cell, is vitally important to maintain cellular functions such as oxygen and electron transport and mitochondrial energy metabolism, whilst preventing permanent cell and tissue damage. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialised proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis. An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimer’s, Parkinson’s, Huntington’s and Multiple Sclerosis (Benarroch 2009). Mouse anti rat CD71 clone OX-26 is reported as suitable for use in immunoelectron microscopy. OX-26 detects a band of ~95kDa in Western blotting under reducing conditions and ~195 kDa under non-reducing conditions reflecting it's homodimeric structure.

WB, IHC-P, Flow Cytometry, IHC-Fr, IF

Flow Cytometry: 1:50 for 1 mg, 0.1 mg, 1:10 for 0.25 mg - 1:200 for 1 mg, 0.1 mg, 1:100 for 0.25 mg, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Rat

PHA activated rat lymphocytes.

Mouse

Monoclonal

OX-26

IgG2a

Unconjugated

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Rat CD71 antibody, clone OX-26 recognizes rat CD71, also known as transferrin receptor, a homodimeric type II transmembrane protein, expressed by all proliferating cells and cells with a requirement for iron, including reticulocytes and capillary endothelium in brain. Clone 0X-26 also binds to a number of non-dividing normal tissues.The balance between a sufficient amount of iron uptake and prevention of accumulation of excess iron within a cell, is vitally important to maintain cellular functions such as oxygen and electron transport and mitochondrial energy metabolism, whilst preventing permanent cell and tissue damage. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialised proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis (Crihton et al. 1992).An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimer’s, Parkinson’s, Huntington’s and Multiple Sclerosis (Benarroch 2009).Mouse anti rat CD71 clone OX-26 is reported as suitable for use in immunoelectron microscopy (Lipardi et al. 2002). OX-26 detects a band of ~95kDa in Western blotting under reducing conditions and ~195 kDa under non-reducing conditions reflecting it's homodimeric structure.

CD 71, CD71, CD71 antigen, IMD46, OTTHUMP00000208523, OTTHUMP00000208524, OTTHUMP00000208525, p90, sTfR, T9, TfR, TFR 1, TfR1, TFR1_HUMAN, TFRC, TR, Transferrin receptor (p90 CD71), Transferrin receptor protein 1, serum form, Trfr