Anti-TRAF4 Antibody (A15508)

Name: Anti-TRAF4 Antibody
Brand: Antibodies.com
SKU: A15508
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to TRAF4 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-TRAF4 Antibody

Description

Rabbit polyclonal antibody to TRAF4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 251-470 of human TRAF4 (NP_004286.2).

Sequence

PFKDSGCKHRCPKLAMARHVEESVKPHLAMMCALVSRQRQELQELRRELEELSVGSDGVLIWKIGSYGRRLQEAKAKPNLECFSPAFYTHKYGYKLQVSAFLNGNGSGEGTHLSLYIRVLPGAFDNLLEWPFARRVTFSLLDQSDPGLAKPQHVTETFHPDPNWKNFQKPGTWRGSLDESSLGFGYPKFISHQDIRKRNYVRDDAVFIRAAVELPRKILS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

54 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CART 1, CART1, cysteine rich domain associated with ring and TRAF domain, Cysteine rich domain associated with RING and Traf domains protein 1, Cysteine-rich domain associated with RING and Traf domains protein 1, Malignant 62, Metastatic lymph node gene 62 protein, MLN 62, MLN62, RING finger protein 83, RNF 83, RNF83, TNF receptor associated factor 4, TNF receptor-associated factor 4, TRAF 4, TRAF4_HUMAN, tumor necrosis receptor-associated factor 4A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (9)

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Figure 1: Western Blot - Anti-TRAF4 Antibody (A15508)

Western blot analysis of extracts of various cell lines, using Anti-TRAF4 Antibody (A15508) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-TRAF4 Antibody (A15508)

Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-TRAF4 Antibody (A15508) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-TRAF4 Antibody (A15508)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-TRAF4 Antibody (A15508) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-TRAF4 Antibody (A15508)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-TRAF4 Antibody (A15508) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.