Anti-TRAF2 Antibody (A84190)

Name: Anti-TRAF2 Antibody
Brand: Antibodies.com
SKU: A84190
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to TRAF2 for ELISA, WB, IHC, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-TRAF2 Antibody

Description

Goat polyclonal antibody to TRAF2.

Applications

ELISA, WB, IHC, IF, Flow Cytometry

Dilutions

ELISA: 1:128,000, WB: 0.1-0.5µg/ml, IHC: 3.75µg/ml, IF: 10µg/ml

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to the C terminal region of human TRAF2.

Sequence

C-KMEAKNSYVRDD

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

50 kDa

Predicted MW

55.9kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

E3 ubiquitin-protein ligase TRAF2, MGC:45012, OTTHUMP00000022625, OTTHUMP00000064745, TNF receptor associated factor 2, TNF receptor-associated factor 2, TNF receptor-associated protein, TRAF 2, TRAF2_HUMAN, TRAP, TRAP 3, TRAP3, Tumor necrosis factor type 2 receptor associated protein 3, Tumor necrosis factor type 2 receptor-associated protein 3

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-TRAF2 Antibody (A84190)

TRAF2 expression in HepG2 (A) cell lysate, Human Ovary (B) lysate, and Jurkat (C) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-TRAF2 Antibody (A84190) at 0.1µg/ml, 0.5µg/ml, or 0.1µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-TRAF2 Antibody (A84190)

TRAF2 expression in Mouse Testes lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-TRAF2 Antibody (A84190) at 0.3µg/ml and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-TRAF2 Antibody (A84190)

TRAF2 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-TRAF2 Antibody (A84190) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and ER staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-TRAF2 Antibody (A84190)

TRAF2 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-TRAF2 Antibody (A84190) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and ER staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.