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Total Protein Extraction Kit for Animal Cultured Cells/Tissues (A319607)

SDS-PAGE - Total Protein Extraction Kit for Animal Cultured Cells/Tissues (A319607) - Antibodies.com Western Blot - Total Protein Extraction Kit for Animal Cultured Cells/Tissues (A319607) - Antibodies.com
$105
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Freight Charges
$40 to United States
Lead Time
7-8 Business Days

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+1 (314) 370-6046
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Name
Total Protein Extraction Kit for Animal Cultured Cells/Tissues
Principle of Assay
Total protein extraction kit for animal cultured cells and tissues is a new generation of ultra fast protein extraction tool. More and more evidence shows that the most commonly used RIPA buffer may lead to random loss of protein, resulting in data that are difficult to explain. The kit uses centrifugal column extraction technology combined with optimized lysis buffer to extract total protein more quickly and effectively, making WB results more accurate. The extraction system can use as little as 20 µL by using a centrifugal column to extract protein, which effectively solves the problem of small sample size. This kit is suitable for applications such as SDS-PAGE, WB and IP.
Sample Type
Animal tissues and cultured cells.
Components
Denaturing cell lysis buffer, Native cell lysis buffer, Protein extraction filter cartridges, Collection tubes with cap, Plastic rods
Storage
Shipped at 4°C. Store at room temperature. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE - Total Protein Extraction Kit for Animal Cultured Cells/Tissues (A319607) - Antibodies.comEnlarge Image
Figure 1: SDS-PAGE - Total Protein Extraction Kit for Animal Cultured Cells/Tissues (A319607)
Protein profiles of mouse liver extracted using denaturing lysis buffer and native buffer from different manufacturers. Extracted proteins were separated on a 10% SDS-PAGE and stained with Coomassie Blue. Lane 1, Molecular weight markers; Lane 2, Native buffer from Manufacturer A; Lane 3, Native buffer from Total Protein Extraction Kit for Animal Cultured Cells/Tissues (A319607); Lane 4, Denaturing lysis buffer from Manufacturer A; Lane 5, Denaturing lysis buffer from Manufacturer B; Lane 6, Denaturing lysis buffer from Manufacturer C; Lane 7, Denaturing lysis buffer from Total Protein Extraction Kit for Animal Cultured Cells/Tissues (A319607); Lane 8, Denaturing lysis buffer from Manufacturer D.
Western Blot - Total Protein Extraction Kit for Animal Cultured Cells/Tissues (A319607) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Total Protein Extraction Kit for Animal Cultured Cells/Tissues (A319607)
Total proteins were extracted from HEK-293 cells and mouse liver using Total Protein Extraction Kit for Animal Cultured Cells/Tissues (A319607) and competitor kits. Lane 1, 5 µL proteins extracted using Manufacturer B kit; Lane 2, 2.5 µL proteins extracted using Manufacturer B kit; Lane 3, 5 µL proteins extracted using Total Protein Extraction Kit for Animal Cultured Cells/Tissues (A319607); Lane 4, 2.5 µL proteins extracted using Total Protein Extraction Kit for Animal Cultured Cells/Tissues (A319607). Samples were analyzed by Western blot, as indicated.
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