Principle of Assay
This kit provides a simple method for detecting the total iron binding capacity (TIBC) in serum samples. The principle is that Fe2+ reacts with ferrozine to form a purple-red compound with a characteristic absorption peak at 562nm. Serum transferrin can be bound to Fe3+ under alkaline conditions, and the remaining unbound Fe3+ can be reduced to Fe2+, at this point, the absorbance A1 is positively correlated with the amount of unbound Fe3+, The bound Fe3+ can be released after acidification, and then be reduced into Fe2+, at this point, absorbance A2 is positively correlated with the total amount of Fe3+. And A2 minus A1 is proportional to the concentration of TIBC.
