Anti-TORC2 Antibody (A84934)

Name: Anti-TORC2 Antibody
Brand: Antibodies.com
SKU: A84934
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to TORC2 for ELISA, WB, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Specifications

Name

Anti-TORC2 Antibody

Description

Goat polyclonal antibody to TORC2.

Applications

ELISA, WB, IF, Flow Cytometry

Dilutions

ELISA: 1:64,000, WB: 1-3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human, Rat

Immunogen

Synthetic peptide corresponding to the C terminal region of mouse TORC2.

Sequence

C-DPAVEDSFRSDRLQ

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

90 kDa

Predicted MW

73.2kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CREB regulated transcription coactivator 2, CREB-regulated transcription coactivator 2, CRTC2, CRTC2_HUMAN, RP11-422P24.6, TORC-2, Transducer of CREB protein 2, Transducer of regulated cAMP response element-binding protein, Transducer of regulated cAMP response element-binding protein (CREB) 2, Transducer of regulated cAMP response element-binding protein 2, Transducer of regulated CREB protein 2

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-TORC2 Antibody (A84934)

TORC2 expression in Jurkat (A) and A431 (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-TORC2 Antibody (A84934) at 1µg/ml (A) or 2µg/ml (B) and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-TORC2 Antibody (A84934)

TORC2 expression in Rat Brain lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-TORC2 Antibody (A84934) at 2µg/ml and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-TORC2 Antibody (A84934)

TORC2 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-TORC2 Antibody (A84934) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Strong nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-TORC2 Antibody (A84934)

TORC2 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-TORC2 Antibody (A84934) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Strong nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.