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Epigenetics & Nuclear Signaling
Chromosome Structure
Scaffold Proteins
Anti-TOP2A (phospho Ser1525) Antibody (A93953)
Overview
Specifications
Images
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Enlarge Image
$185
Product Datasheet
Rabbit polyclonal antibody to TOP2A (phospho Ser1525) for WB, IHC and ELISA.
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100µg
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Specifications
Name
Anti-TOP2A (phospho Ser1525) Antibody
Description
Rabbit polyclonal antibody to TOP2A (phospho Ser1525).
Specificity
This antibody detects endogenous levels of TOP2A only when phosphorylated at Ser1525.
Applications
WB
,
IHC
,
ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phospho-peptide derived from human TOP2A around the phosphorylation site of Ser1525.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
174kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Rabbit IgG (A82272)
Rabbit IgG (A17360)
Suitable Secondaries
Goat Anti-Rabbit IgG H&L Antibody (AP) (A294874)
Goat Anti-Rabbit IgG H&L Antibody (Biotin) (A294795)
Goat Anti-Rabbit IgG H&L Antibody (FITC) (A294887)
Goat Anti-Rabbit IgG H&L Antibody (HRP) (A294888)
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Scientific Validation Data (2)
Enlarge Image
Figure 1: Anti-TOP2A (phospho Ser1525) Antibody (A93953)
ELISA for immunogen phosphopeptide (left) and non-phosphopeptide (right) using Anti-TOP2A (phospho Ser1525) Antibody.
Enlarge Image
Figure 2: Western Blot - Anti-TOP2A (phospho Ser1525) Antibody (A93953)
Western blot analysis of lysates from RAW264.7 cells treated with TNF 20ng/ml 5' using Anti-TOP2A (phospho Ser1525) Antibody.
Publishing research using Anti-TOP2A (phospho Ser1525) Antibody (A93953)? Please
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