Recombinant Anti-TOP1MT Antibody [rTOP1MT/488] (A277880)

Anti-TOP1MT Antibody [rTOP1MT/488]

Recombinant mouse monoclonal [rTOP1MT/488] antibody to TOP1MT.

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

Flow Cytometry, IF, WB, IHC-P

Flow Cytometry: 0.5-1 µg/million cells, IF: 0.5-1 µg/ml, WB: 0.5-2 µg/ml, IHC-P: 1-2 µg/ml

Human

Recombinant full-length human TOP1MT protein.

Mouse

Monoclonal

rTOP1MT/488

IgG1

kappa

Protein A/G chromatography.

200 µg/ml

70 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-TOP1MT Antibody [rTOP1MT/488] - BSA and Azide free (A278468).

2900052H09Rik, DNA topoisomerase I, DNA topoisomerase I, mitochondrial, mitochondrial, TOP1M_HUMAN, Topoisomerase I mitochondrial