Anti-TNF Receptor I Antibody (A13494)

Name: Anti-TNF Receptor I Antibody
Brand: Antibodies.com
SKU: A13494
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to TNF Receptor I for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-TNF Receptor I Antibody

Description

Rabbit polyclonal antibody to TNF Receptor I.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 345-455 of human TNFR1/TNFRSF1A (NP_001056.1).

Sequence

AHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CD120a, p55, p60, TNF-R1, TNF-RI, TNFAR, TNFR-I, TNFRSF1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor type I

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-TNF Receptor I Antibody (A13494)

Western blot analysis of extracts of various cell lines, using Anti-TNF Receptor I Antibody (A13494) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-TNF Receptor I Antibody (A13494)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-TNF Receptor I Antibody (A13494) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-TNF Receptor I Antibody (A13494)

Immunofluorescence analysis of BALB-3T3 cells using Anti-TNF Receptor I Antibody (A13494) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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