Western Blot - Anti-TNF Receptor I Antibody (A305105)
Western blot analysis of mouse liver cell lysates showing detection of ~55 kDa TNF-R1 protein using Anti-TNF Receptor I Antibody (A305105) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~55 kDa.
Western Blot - Anti-TNF Receptor I Antibody (A305105)
Western blot analysis of human A549 showing detection of ~ 50 kDa TNF-R1 protein using Anti-TNF Receptor I Antibody (A305105) at 1:1,000 for 2 hours at room temperature with shaking. Lane 1: MW Ladder, Lane 2: A549. Load: 30 ug. Block: 5% BSA in TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:4,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature. Predicted/Observed Size: ~ 50 kDa. Other Band(s): ~90-100kDa. Other bands can be explained by a few factors, such as oligomerization, self-aggregation, cleavage of the TNFR1 extracellular domain, etc.( Literature references: doi: 10.3389/fcell.2020.615141, 10.1128/MCB.22.8.2536-2543.2002, 10.1073/pnas.0307981100).
Immunofluorescence - Anti-TNF Receptor I Antibody (A305105)
Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-TNF Receptor I Antibody (A305105), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Golgi apparatus membrane. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-TNF-R1 Antibody. (C) Composite.
Immunofluorescence - Anti-TNF Receptor I Antibody (A305105)
Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol at -20°C for 10 minutes, using Anti-TNF Receptor I Antibody (A305105), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at room temperature in dark. Localization: Punctate nuclear staining, dotty staining in cytoplasm.
Immunohistochemistry - Anti-TNF Receptor I Antibody (A305105)
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-TNF Receptor I Antibody (A305105) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: dermis.
Immunofluorescence - Anti-TNF Receptor I Antibody (A305105)
Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-TNF Receptor I Antibody (A305105), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Golgi apparatus membrane. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-TNF-R1 Antibody. (C) Composite.
