Anti-TNF alpha Antibody (Biotin) - BSA and Azide free (A282245)

$625

Rabbit polyclonal antibody to TNF alpha (Biotin) for WB and ELISA.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-TNF alpha Antibody (Biotin) - BSA and Azide free
Description
Rabbit polyclonal antibody to TNF alpha (Biotin).
Specificity
This antibody recognises rat tumor necrosis factor alpha (TNFa), also known as Cachectin or Tumor necrosis factor ligand superfamily member 2. Rat TNFa is a 235 amino acid ~17 kDa single pass type II O-linked transmembrane glycoprotein, it also exists as a secreted cytokine through cleavage between residues 79 and 80. TNFa is produced by many cell types including macrophages, monocytes, neutrophils and a population of activated NK cells.
Applications
WB, ELISA
Dilutions
ELISA: 0.15 - 0.30 µg/ml, WB: 0.1 - 0.2 µg/ml
Reactivity
Rat
Immunogen
Recombinant rat TNF alpha.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Biotin

Concentration
100 µg/ml after reconstitution.
Product Form
Lyophilized
Reconstitution
Reconstitute with 0.5 ml of sterile Phosphate Buffered Saline containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti Rat TNF alpha antibody recognizes rat tumor necrosis factor alpha (TNFa), also known as Cachectin or Tumor necrosis factor ligand superfamily member 2. Rat TNFa is a 235 amino acid ~17 kDa single pass type II O-linked transmembrane glycoprotein, it also exists as a secreted cytokine through cleavage between residues 79 and 80. TNFa is produced by many cell types including macrophages, monocytes, neutrophils and a population of activated NK cells.
Synonyms
APC1, APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, soluble form, Tnf, TNF a, TNF macrophage derived, TNF monocyte derived, TNF superfamily member 2 , TNF superfamily, member 2, TNF, macrophage derived, TNF, monocyte derived, TNF-a, TNF-alpha, TNFA, TNFA_HUMAN, TNFSF2, Tumor necrosis factor, Tumor necrosis factor (TNF superfamily member 2) , Tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor, Membrane Form, Tumor necrosis factor, soluble form
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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