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TMPRSS3 Cell Based ELISA Kit (A103404)

Western Blot - TMPRSS3 Cell Based ELISA Kit (CB5932) - Antibodies.com Protocol Diagram - TMPRSS3 Cell Based ELISA Kit (CB5932) - Antibodies.com Protocol Summary - TMPRSS3 Cell Based ELISA Kit (CB5932) - Antibodies.com Western Blot - Control Antibody - TMPRSS3 Cell Based ELISA Kit (CB5932) - Antibodies.com
$550
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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
TMPRSS3 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This TMPRSS3 Cell Based ELISA Kit allows for the detection of TMPRSS3 and the effects that certain stimulation conditions have on TMPRSS3 expression in different cell lines. Qualitative determination of TMPRSS3 concentration is achieved by an indirect ELISA format. In essence, the TMPRSS3 is captured by Anti-TMPRSS3 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this TMPRSS3 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
Deafness autosomal recessive 10, DFNB10, DFNB8, ECHOS1, Gene similar to transmembrane serine protease, MGC130589, Serine protease TADG-12, si:dz69g10.3, TADG12, TMPRSS 3, TMPS3_HUMAN, Transmembrane protease serine 3, Tumor associated differentially expressed gene 12 protein, Tumor-associated differentially-expressed gene 12 protein, UNQ323/PRO382
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - TMPRSS3 Cell Based ELISA Kit (CB5932) - Antibodies.comEnlarge Image
Figure 1: Western Blot - TMPRSS3 Cell Based ELISA Kit (A103404)
Western blot analysis of extracts from HuvEc cells, using Anti-TMPRSS3 Antibody.
Protocol Diagram - TMPRSS3 Cell Based ELISA Kit (CB5932) - Antibodies.comEnlarge Image
Figure 2: TMPRSS3 Cell Based ELISA Kit (A103404)
A diagram showing the protocol for TMPRSS3 Cell Based ELISA Kit.
Protocol Summary - TMPRSS3 Cell Based ELISA Kit (CB5932) - Antibodies.comEnlarge Image
Figure 3: TMPRSS3 Cell Based ELISA Kit (A103404)
A summary of the protocol for TMPRSS3 Cell Based ELISA Kit.
Western Blot - Control Antibody - TMPRSS3 Cell Based ELISA Kit (CB5932) - Antibodies.comEnlarge Image
Figure 4: Western Blot - TMPRSS3 Cell Based ELISA Kit (A103404)
The mouse monoclonal antibody to GAPDH is used as a positive control inTMPRSS3 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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