Figure 1: Western Blot - Anti-TLK2 Antibody (A15369)
Western blot analysis of extracts of various cell lines, using Anti-TLK2 Antibody (A15369) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Figure 2: Western Blot - Anti-TLK2 Antibody (A15369)
Western blot analysis of extracts from normal (control) and TLK2 Rabbit polyclonal antibody knockout (KO) NIH/3T3 cells, using Anti-TLK2 Antibody (A15369) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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