Principle of Assay
Tissue - Slide Adhesive reacts with silica glass to change its surface chemistry so that it may bond either ionically or covalently with aldehyde and ketone groups in tissue sections. Proper tissue adhesion to glass slides is a critical step in immunohistochemistry. Good immunostaining may require harsh pretreatment of tissues for antigen retrieval (e.g. proteolytic enzyme digestion, boiling in high pH buffer, treatment with detergents or saponin). Improper adhesion of tissue sections can lead to poor quality staining or even loss of tissue sections. White glue, albumen, chrome-gelatin, and poly-L-lysine increase background staining. There is a need for a tissue adhesive that can irreversibly bind tissue sections to glass without creating background staining. Glass treated with Tissue - Slide Adhesive can be used for both paraffin and frozen tissue sections.
