Anti-TIMP2 Antibody (A13504)

Name: Anti-TIMP2 Antibody
Brand: Antibodies.com
SKU: A13504
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to TIMP2 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-TIMP2 Antibody

Description

Rabbit polyclonal antibody to TIMP2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human TIMP2 (NP_003246.1).

Sequence

VSEKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPEKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITLCDFIVPWDTLSTTQKKSLNHRYQM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

24 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CSC 21K , CSC-21K, CSC21K , D11Bwg1104e, DDC8, Metalloproteinase inhibitor 2 , Metalloproteinase inhibitor 2 precursor , MGC105282, OTTMUSP00000004170, OTTMUSP00000004171, RP23-394O9.2, TIMP 2 , TIMP metallopeptidase inhibitor 2 , TIMP-2, TIMP2_HUMAN, Tissue inhibitor of metalloproteinase 2, Tissue inhibitor of metalloproteinases 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-TIMP2 Antibody (A13504)

Western blot analysis of extracts of various cell lines, using Anti-TIMP2 Antibody (A13504) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-TIMP2 Antibody (A13504)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-TIMP2 Antibody (A13504) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-TIMP2 Antibody (A13504)

Immunofluorescence analysis of C6 cells using Anti-TIMP2 Antibody (A13504) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-TIMP2 Antibody (A13504)

Immunofluorescence analysis of NIH/3T3 cells using Anti-TIMP2 Antibody (A13504) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).