Anti-TIM50 Antibody [ARC1883] (A306816)

Name: Anti-TIM50 Antibody [ARC1883]
Brand: Antibodies.com
SKU: A306816
Price: 485.0 USD
Availability: InStock

Enlarge Image

$485

Rabbit monoclonal [ARC1883] antibody to TIM50 for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-TIM50 Antibody [ARC1883]

Description

Rabbit monoclonal [ARC1883] antibody to TIM50.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TIMM50 (Q3ZCQ8).

Sequence

MAASAAVFSRLRSGLRLGSRGLCTRLATPPRRAPDQAAEIGSRGSTKAQGPQQQPGSEGPSYAKKVALWLAGLLGAGGTVSVVYIFGNNPVDENGAKIPD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1883

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

38 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

Enlarge Image

Western Blot - Anti-TIM50 Antibody [ARC1883] (A306816)

Western blot analysis of extracts of various cell lines, using Anti-TIM50 Antibody [ARC1883] (A306816) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Western Blot - Anti-TIM50 Antibody [ARC1883] (A306816)

Enlarge Image

Immunohistochemistry - Anti-TIM50 Antibody [ARC1883] (A306816)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-TIM50 Antibody [ARC1883] (A306816) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Enlarge Image

Immunohistochemistry - Anti-TIM50 Antibody [ARC1883] (A306816)

Immunohistochemistry analysis of paraffin-embedded mouse pancreas using Anti-TIM50 Antibody [ARC1883] (A306816) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.