Anti-TIM 3 Antibody (A84174)

Name: Anti-TIM 3 Antibody
Brand: Antibodies.com
SKU: A84174
Price: 480.0 USD
Availability: InStock

This product is identical to Abcam (ab47997). Discover more for less.

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$480

Goat polyclonal antibody to TIM 3 for ELISA, WB, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-TIM 3 Antibody

Description

Goat polyclonal antibody to TIM 3.

Applications

ELISA, WB, IF, Flow Cytometry

Dilutions

ELISA: 1:64,000, WB: 0.1-1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human TIM 3.

Sequence

C-KWYSHSKEKIQN

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

35 kDa

Predicted MW

33.4kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CD366, FLJ14428, HAVcr-2, Havcr2, HAVR2_HUMAN, Hepatitis A virus cellular receptor 2, Kidney injury molecule 3, KIM 3, KIM3, T cell immunoglobulin and mucin domain containing 3, T cell immunoglobulin mucin 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin family member 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM-3, TIM3, TIMD-3, TIMD3

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-TIM 3 Antibody (A84174)

Anti-HAVCR2 Antibody (A84174) (2µg/ml) staining of Jurkat (A), MOLT4 (B) and negative control A431 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

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Figure 2: Western Blot - Anti-TIM 3 Antibody (A84174)

Anti-HAVCR2 Antibody (A84174) (1µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-TIM 3 Antibody (A84174)

Anti-HAVCR2 Antibody (A84174) - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing membrane/cytoplasmic and nuclear staining.

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Figure 4: Flow Cytometry - Anti-TIM 3 Antibody (A84174)

Anti-HAVCR2 Antibody (A84174) - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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