Anti-TIM 1 Antibody [RMT1-10] (A281685)

Anti-TIM 1 Antibody [RMT1-10]

Rat monoclonal [RMT1-10] antibody to TIM 1.

This antibody recognises mouse Tim-1 (T-cell immunoglobulin mucin 1), a cell surface glycoprotein first identified as the hepatitis A virus cellular receptor (HAVCR). TIM-1 is highly expressed in the liver (isoform Tim-1a) and the kidneys (isoform Tim-1b).Tim-1 is highly expressed by activated CD4+ T cells, acting as a positive/negative co-stimulatory molecule of T cell proliferation, cytokine production and tolerance abrogation, and is the receptor for Tim-4, expressed on APCs. Tim-1 expression is greater on Th2 than Th1 cells, interaction between Tim-1 on Th2 cells and Tim-4 on dendritic cells (DCs), enhances Th2 cell function, and have implicated Tim-1 as a critical player in the development of atopic disease, and in particular airway hypersensitivity.In humans, polymorphic forms of Tim-1 are associated with increased susceptibility to asthma, eczema and rheumatoid arthritis. Rat anti Mouse TIM-1 antibody, clone RMT1-10 promotes Th2 responses, and inhibits antigen-specific T cell proliferation, in contrast to the agonistic function of many Tim-1 antibodies. Rat anti Mouse TIM-1 antibody, clone RMT1-10 reduces the severity of experimental autoimmune encephalomyelitis and delays disease onset in mice.

Flow Cytometry, ELISA, IF

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Mouse

Full length mouse Tim-1-Ig, containing both the IgV and mucin domains of Tim-1.

Rat

Monoclonal

RMT1-10

IgG2a

Unconjugated

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse TIM-1 antibody, clone RMT1-10 recognizes mouse Tim-1 (T-cell immunoglobulin mucin 1), a cell surface glycoprotein first identified as the hepatitis A virus cellular receptor (HAVCR). TIM-1 is highly expressed in the liver (isoform Tim-1a) and the kidneys (isoform Tim-1b).Tim-1 is highly expressed by activated CD4+ T cells, acting as a positive/negative co-stimulatory molecule of T cell proliferation, cytokine production and tolerance abrogation, and is the receptor for Tim-4, expressed on APCs. Tim-1 expression is greater on Th2 than Th1 cells, interaction between Tim-1 on Th2 cells and Tim-4 on dendritic cells (DCs), enhances Th2 cell function, and have implicated Tim-1 as a critical player in the development of atopic disease, and in particular airway hypersensitivity.In humans, polymorphic forms of Tim-1 are associated with increased susceptibility to asthma, eczema and rheumatoid arthritis (Rodriguez-Manzanet et al. 2009).Rat anti Mouse TIM-1 antibody, clone RMT1-10 promotes Th2 responses, and inhibits antigen-specific T cell proliferation, in contrast to the agonistic function of many Tim-1 antibodies (Xiao et al. 2007). Rat anti Mouse TIM-1 antibody, clone RMT1-10 reduces the severity of experimental autoimmune encephalomyelitis and delays disease onset in mice (Xiao et al. 2007).

CD365, HAVCR, HAVCR 1, HAVcr-1, Havcr1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, KIM 1, KIM-1, T cell immunoglobin domain and mucin domain protein 1, T cell immunoglobulin mucin family member 1, T cell immunoglobulin mucin receptor 1, T-cell immunoglobulin and mucin domain-containing protein 1, T-cell membrane protein 1, TIM, TIM-1, TIM1, TIMD 1, TIMD-1, TIMD1, TIMD1_HUMAN