TIE2 (phospho Tyr1108) Cell Based ELISA Kit (A103810)

$645
Freight Charges
Lead Time
5-8 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
TIE2 (phospho Tyr1108) Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This TIE2 (phospho Tyr1108) Cell Based ELISA Kit allows for the detection of TIE2 (phospho Tyr1108) and the effects that certain stimulation conditions have on TIE2 (phospho Tyr1108) expression in different cell lines. Qualitative determination of TIE2 (phospho Tyr1108) concentration is achieved by an indirect ELISA format. In essence, TIE2 (phospho Tyr1108) is captured by Anti-TIE2 (phospho Tyr1108) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-TIE2 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.
Platform
Microplate
Detection Type
Fluorometric
Product Range
> 5000 Cells
Reactivity
Human, Mouse
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202b, CD202b antigen, EC 2.7.10.1, Endothelial tyrosine kinase, Endothelium specific receptor tyrosine kinase 2, hTIE 2, hTIE2, Hyk, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, Tek, tek tyrosine kinase, TEK tyrosine kinase endothelial, TEK tyrosine kinase, endothelial, TIE 2, TIE2_HUMAN, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains 2, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine protein kinase receptor TEK, Tyrosine protein kinase receptor TIE 2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE 2, Tyrosine-protein kinase receptor TIE-2, Venous malformations multiple cutaneous and mucosal, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM 1, VMCM1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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