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Anti-Thyroid Peroxidase/TPO Antibody (A83130)

Western Blot - Anti-TPO Antibody (A83130) - Antibodies.com Immunohistochemistry - Anti-TPO Antibody (A83130) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to Thyroid Peroxidase/TPO for ELISA, WB and IHC.

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Freight Charges
$40 to United States
Lead Time
3-5 Business Days

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Name
Anti-Thyroid Peroxidase/TPO Antibody
Description
Goat polyclonal antibody to Thyroid Peroxidase/TPO.
Applications
ELISA, WB, IHC
Dilutions
ELISA: 1:32,000, WB: 0.1-0.3µg/ml, IHC: 5-10µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C terminal region of human Thyroid Peroxidase/TPO.
Sequence
ELRCGKHQAVGTSPQ
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
110 kDa
Predicted MW
103kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-TPO Antibody (A83130) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Thyroid Peroxidase/TPO Antibody (A83130)
Anti-TPO Antibody (A83130) (0.1µg/ml) staining of Thyroid Gland lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry - Anti-TPO Antibody (A83130) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Thyroid Peroxidase/TPO Antibody (A83130)
Anti-TPO Antibody (A83130) (5µg/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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