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Anti-Thyroid Hormone Receptor alpha Antibody (A14855)

Western Blot - Anti-Thyroid Hormone Receptor alpha Antibody (A14855) - Antibodies.com Immunohistochemistry - Anti-Thyroid Hormone Receptor alpha Antibody (A14855) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Thyroid Hormone Receptor alpha for WB and IHC.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Thyroid Hormone Receptor alpha Antibody
Description
Rabbit polyclonal antibody to Thyroid Hormone Receptor alpha.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence within amino acids 119-199 of human THRA (NP_003241.2).
Sequence
GMAMDLVLDDSKRVAKRKLIEQNRERRRKEEMIRSLQQRPEPTPEEWDLIHIATEAHRSTNAQGSHWKQRRKFLPDDIGQS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AR7, c-erbA-1, C-erbA-alpha, CHNG6, EAR-7, EAR7, ERB-T-1, ERBA, ERBA-related 7, ERBA1, NR1A1, Nuclear receptor subfamily 1 group A member 1, THA_HUMAN, THRA, THRA1, THRA2, thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian), thyroid normone nuclear receptor alpha variant 1, triiodothyronine receptor, V-erbA-related protein 7
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Thyroid Hormone Receptor alpha Antibody (A14855) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Thyroid Hormone Receptor alpha Antibody (A14855)
Western blot analysis of extracts of SH-SY5Y cells, using Anti-Thyroid Hormone Receptor alpha Antibody (A14855) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-Thyroid Hormone Receptor alpha Antibody (A14855) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Thyroid Hormone Receptor alpha Antibody (A14855)
Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-Thyroid Hormone Receptor alpha Antibody (A14855) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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