Mouse monoclonal [1aG4/C5] antibody to Thy1.2 (PE).
Specificity
This antibody recognizes alloantigen Thy-1.2 of mouse Thy-1. This antibody does not cross-react with Thy-1.1 alloantigen. Thy-1 is an 18-35 kDa cell surface GPI linked glycoprotein (immunoglobulin superfamily); in mouse it is abundant on thymocytes and peripheral T lymphocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1-2 µg/ml.
Reactivity
Mouse
Cross Reactivity
This antibody does not cross react with Rat or Human.
Immunogen
Thymocytes from C3H/Di mice (Thy-1.2 positive).
Host
Mouse
Clonality
Monoclonal
Clone ID
1aG4/C5
Isotype
IgG3
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
100 µg/ml
Predicted MW
18-35 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!