Anti-Thrombospondin 1 Antibody (A11512)

Name: Anti-Thrombospondin 1 Antibody
Brand: Antibodies.com
SKU: A11512
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Thrombospondin 1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Thrombospondin 1 Antibody

Description

Rabbit polyclonal antibody to Thrombospondin 1.

Applications

WB, IHC

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 19-352 of human THBS1 (NP_003237.2).

Sequence

NRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

160 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

THBS1, TSP, Tsp1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Thrombospondin 1 Antibody (A11512)

Western blot analysis of extracts of Mouse liver, using Anti-Thrombospondin 1 Antibody (A11512) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-Thrombospondin 1 Antibody (A11512)

Western blot analysis of extracts of Rat lung, using Anti-Thrombospondin 1 Antibody (A11512) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-Thrombospondin 1 Antibody (A11512)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Thrombospondin 1 Antibody (A11512) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Thrombospondin 1 Antibody (A11512)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Thrombospondin 1 Antibody (A11512) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.