Anti-Thrombin Aptamer [T7] (Biotin) (A321249)

Freight Charges
Lead Time
30-45 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-Thrombin Aptamer [T7] (Biotin)
Description
Synthetic DNA aptamer to Thrombin [T7] (Biotin).
Specificity
Shows no Binding against other proteins (IL-10, TNF, IFN, IL-7, HSA, IL-12).
Aptamer ID
T7
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatamer Refolding Conditions
Denatured at 95°C for 5 min and then cooled for 10 min.
Apatmer Binding Conditions
Phosphate Buffered Saline, with 5mM Magnesium Chloride, 1mM Calcium Chloride, and 2.5mM Potassium Chloride. Binding Temperature: 25°C.
Sequence
5'-dCpdApdCpdCpdTpdApdApdTpdApdCpdGpdApdCpdTpdCpdApdCpdTpdApdTpdApdGpdCpdGpdGpdApdTpdCpdCpdGpdApdCpdTpdApdApdApdGpdApdApdTpdApdGpdGpdTpdGpdApdTpdGpdGpdGpdGpdApdTpdApdTpdGpdGpdGpdApdTpdCpdApdTpdApdGpdGpdTpdGpdCpdApdCpdCpdTpdGpdGpdCpdTpdCpdGpdApdApdCpdApdApdGpdCpdTpdTpdGpdCp-3'
Aptamer Length
90 nt
Aptamer Molecular Weight (Da)
29,700 Da
Aptamer Affinity
Affinity (Kd) = 1.5 nM
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Thrombin Aptamer [T7] (Biotin) (A321249) is a replica of the aptamer described in PMID: 37798416.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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