Anti-Thrombin Aptamer [CTBA4T-B1] (Biotin) (A321198)

Freight Charges
Lead Time
30-45 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-Thrombin Aptamer [CTBA4T-B1] (Biotin)
Description
Synthetic DNA aptamer to Thrombin [CTBA4T-B1] (Biotin).
Aptamer ID
CTBA4T-B1
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Binding Buffer: Phosphate Buffered Saline, pH 7.2, with 137mM Sodium Chloride, 2.68mM Potassium Chloride, 8.1mM Disodium Phosphate, 1.76mM Potassium Phosphate, 1mM Magnesium Chloride, and 0.025% Tween 20. Binding Temperature: 95°C, 25°C.
Sequence
5'-dApdTpdCpdTpdCpdGpdApdGpdTpdCpdApdTpdApdGpdGpdGpdGpdGpdCpdGpdCpdGpdApdApdCpdApdTpdApdCpdGpdCpdGpdGpdTpdTpdGpdGpdTpdGpdTpdGpdGpdTpdTpdGpdGpdCpdTpdGpdApdCpdTpdCpdGpdTp-3'
Aptamer Length
55 nt
Aptamer Molecular Weight (Da)
17,110.1 Da
Aptamer Affinity
Affinity (Kd) = 19 pM
Extinction Coefficient
525200 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Thrombin Aptamer [CTBA4T-B1] (Biotin) (A321198) is a replica of the aptamer described in PMID: 33021630.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Anti-Thrombin Aptamer [CTBA4T-B1] (Biotin) (A321198)? Please let us know so that we can list the citation on this page.
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