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TGF beta 1 Cell Based ELISA Kit (A102901)

Western Blot - TGF beta 1 Cell Based ELISA Kit (CB5683) - Antibodies.com Protocol Diagram - TGF beta 1 Cell Based ELISA Kit (CB5683) - Antibodies.com Protocol Summary - TGF beta 1 Cell Based ELISA Kit (CB5683) - Antibodies.com Western Blot - Control Antibody - TGF beta 1 Cell Based ELISA Kit (CB5683) - Antibodies.com
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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
TGF beta 1 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This TGF beta 1 Cell Based ELISA Kit allows for the detection of TGF beta 1 and the effects that certain stimulation conditions have on TGF beta 1 expression in different cell lines. Qualitative determination of TGF beta 1 concentration is achieved by an indirect ELISA format. In essence, the TGF beta 1 is captured by Anti-TGF beta 1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this TGF beta 1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
Camurati-Engelmann disease, Cartilage-inducing factor, CED, Differentiation inhibiting factor, DPD1, LAP, Latency-associated peptide, Prepro transforming growth factor beta 1, TGF beta, TGF beta 1 protein , TGF-beta 1 protein, TGF-beta-1, TGF-beta-5, TGF-beta1, TGFB, Tgfb-1, tgfb1, TGFB1_HUMAN, TGFbeta, TGFbeta1, Transforming Growth Factor b1, Transforming growth factor beta 1, Transforming growth factor beta 1a, transforming growth factor beta-1, transforming growth factor, beta 1, Transforming Growth Factor-ß1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - TGF beta 1 Cell Based ELISA Kit (CB5683) - Antibodies.comEnlarge Image
Figure 1: Western Blot - TGF beta 1 Cell Based ELISA Kit (A102901)
Western blot analysis of extracts from HepG2 cells, using Anti-TGF beta1 Antibody.
Protocol Diagram - TGF beta 1 Cell Based ELISA Kit (CB5683) - Antibodies.comEnlarge Image
Figure 2: TGF beta 1 Cell Based ELISA Kit (A102901)
A diagram showing the protocol for TGF beta 1 Cell Based ELISA Kit.
Protocol Summary - TGF beta 1 Cell Based ELISA Kit (CB5683) - Antibodies.comEnlarge Image
Figure 3: TGF beta 1 Cell Based ELISA Kit (A102901)
A summary of the protocol for TGF beta 1 Cell Based ELISA Kit.
Western Blot - Control Antibody - TGF beta 1 Cell Based ELISA Kit (CB5683) - Antibodies.comEnlarge Image
Figure 4: Western Blot - TGF beta 1 Cell Based ELISA Kit (A102901)
The mouse monoclonal antibody to GAPDH is used as a positive control inTGF beta 1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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