This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Applications
ELISA, IF, IHC-P
Dilutions
IF: 1-2 µg/ml, IHC-P: 2-4 µg/ml
Reactivity
Human, Bovine, Rat, Mouse
Immunogen
Synthetic peptide corresponding to 17 amino acids from the C terminal of rat TGF alpha. The exact sequence is proprietary.
Host
Mouse
Clonality
Monoclonal
Clone ID
MF9
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
~6 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-TGF alpha Antibody [MF9] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Publishing research using Anti-TGF alpha Antibody [MF9] (A250114)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-TGF alpha Antibody [MF9] (A250114)