Mouse monoclonal [PCRP-GTF2B-1D1] antibody to TFIIB.
Specificity
In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the TATA element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.
Flow cytometric analysis of PFA-fixed HeLa cells using Anti-TFIIB Antibody [PCRP-GTF2B-1D1] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).
SDS-PAGE analysis of Anti-TFIIB Antibody [PCRP-GTF2B-1D1] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-TFIIB Antibody [PCRP-GTF2B-1D1]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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