Anti-TFE3 Antibody [ARC1829] (A306694)

Name: Anti-TFE3 Antibody [ARC1829]
Brand: Antibodies.com
SKU: A306694
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1829] antibody to TFE3 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-TFE3 Antibody [ARC1829]

Description

Rabbit monoclonal [ARC1829] antibody to TFE3.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 191-440 of human TFE3 (P19532).

Sequence

LQQARRQQVKQYLSTTLGPKLASQALTPPPGPASAQPLPAPEAAHTTGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTTGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSSDPEMRWNKGTILKASVDYIRKLQKEQQRSKDLESRQRSLEQANRSLQLRIQELELQAQIHGLP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1829

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

bHLHe33, Class E basic helix-loop-helix protein 33, Transcription factor E3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-TFE3 Antibody [ARC1829] (A306694)

Western blot analysis of extracts of various cell lines, using Anti-TFE3 Antibody [ARC1829] (A306694) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-TFE3 Antibody [ARC1829] (A306694)

Western blot analysis of extracts of HeLa cells, using Anti-TFE3 Antibody [ARC1829] (A306694) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-TFE3 Antibody [ARC1829] (A306694)

Immunohistochemistry analysis of paraffin-embedded human acinar soft tissue sarcoma using Anti-TFE3 Antibody [ARC1829] (A306694) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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