Rat monoclonal [91a] antibody to TCP1 alpha / CCTA.
Specificity
This antibody recognises weakly Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown to recognise with a subunit of a specialized chaperonin which folds phytochrome.
Applications
WB, ICC/IF, IP, Flow Cytometry
Dilutions
WB: 1:1,000, ICC/IF: 1:100
Reactivity
Human, Mouse, Rat, Bovine, Rabbit, Guinea Pig, Hamster, Canine, Porcine, Yeast, C. elegans, Drosophila, Monkey
Cross Reactivity
This antibody cross-reacts with human HSP60 in immunoblot analysis.
Immunogen
Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region)
Host
Rat
Clonality
Monoclonal
Clone ID
91a
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein G purification.
Concentration
1 mg/ml
Molecular Weight
~60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-TCP1 alpha / CCTA Antibody [91a] (A304713), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rat (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Centrosome. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Figure 2: Western Blot - Anti-TCP1 alpha / CCTA Antibody [91a] (A304713)
Western blot analysis of human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein using Anti-TCP1 alpha / CCTA Antibody [91a] (A304713) at 1:1,000.
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-TCP1 alpha / CCTA Antibody [91a] (A304713), at 1:100 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Centrosome. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
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![Immunocytochemistry/Immunofluorescence - Anti-TCP1 alpha / CCTA Antibody [91a] (A304713) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304713_1.png?profile=product_top)
![Western Blot - Anti-TCP1 alpha / CCTA Antibody [91a] (A304713) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304713_2.png?profile=product_top)
![Immunocytochemistry/Immunofluorescence - Anti-TCP1 alpha / CCTA Antibody [91a] (A304713) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304713_3.png?profile=product_top)
![Immunocytochemistry/Immunofluorescence - Anti-TCP1 alpha / CCTA Antibody [91a] (A304713) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304713_1.png?profile=product_top_thumb)
![Western Blot - Anti-TCP1 alpha / CCTA Antibody [91a] (A304713) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304713_2.png?profile=product_top_thumb)
![Immunocytochemistry/Immunofluorescence - Anti-TCP1 alpha / CCTA Antibody [91a] (A304713) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304713_3.png?profile=product_top_thumb)
![Immunocytochemistry/Immunofluorescence - Anti-TCP1 alpha / CCTA Antibody [91a] (A304713) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304713_1.png?profile=product_image)
![Western Blot - Anti-TCP1 alpha / CCTA Antibody [91a] (A304713) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304713_2.png?profile=product_image)
![Immunocytochemistry/Immunofluorescence - Anti-TCP1 alpha / CCTA Antibody [91a] (A304713) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304713_3.png?profile=product_image)