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Anti-TCF3 Antibody (A82557)

Western Blot - Anti-TCF3 Antibody (A82557) - Antibodies.com Immunohistochemistry - Anti-TCF3 Antibody (A82557) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to TCF3 for ELISA, WB and IHC.

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Alternative Formats and ConjugatesAlternative Formats

Freight Charges
$40 to United States
Lead Time
3-5 Business Days

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Name
Anti-TCF3 Antibody
Description
Goat polyclonal antibody to TCF3.
Applications
ELISA, WB, IHC
Dilutions
ELISA: 1:128,000, WB: 0.01-0.03µg/ml, IHC: 3-5µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the internal region of human TCF3.
Sequence
C-KAPRARTSPDEDED
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
75 kDa
Predicted MW
67.6kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-TCF3 Antibody (A82557) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-TCF3 Antibody (A82557)
TCF3 expression in Daudi cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-TCF3 Antibody (A82557) at 0.01µg/ml and detected by chemiluminescence.
Immunohistochemistry - Anti-TCF3 Antibody (A82557) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-TCF3 Antibody (A82557)
TCF3 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-TCF3 Antibody (A82557) at 3.8µg/ml and revealed with alkaline phosphatase (AP).
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