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Anti-Tau (phospho Ser404) Antibody [ARC58154] (A309488)

Western Blot - Anti-Tau (phospho Ser404) Antibody [ARC58154] (A309488) - Antibodies.com Western Blot - Anti-Tau (phospho Ser404) Antibody [ARC58154] (A309488) - Antibodies.com
$540
Product Datasheet

Rabbit monoclonal [ARC58154] antibody to Tau (phospho Ser404) for WB.

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Name
Anti-Tau (phospho Ser404) Antibody [ARC58154]
Description
Rabbit monoclonal [ARC58154] antibody to Tau (phospho Ser404).
Applications
WB
Dilutions
WB: 1:2,000-1:6,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around S404 of human Tau.
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC58154
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
50 - 80 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AI413597, AW045860, DDPAC, FLJ31424, FTDP 17, G protein beta1/gamma2 subunit interacting factor 1, MAPT, MAPTL, MGC134287, MGC138549, MGC156663, Microtubule associated protein tau, Microtubule associated protein tau isoform 4, Microtubule-associated protein tau, MSTD, Mtapt, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, Paired helical filament-tau, PHF tau, PHF-tau, PPND, PPP1R103, Protein phosphatase 1, regulatory subunit 103, pTau, RNPTAU, Tauopathy and respiratory failure, included, TAU_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Tau (phospho Ser404) Antibody [ARC58154] (A309488) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Tau (phospho Ser404) Antibody [ARC58154] (A309488)
Western blot analysis of SH-SY5Y, using Anti-Tau (phospho Ser404) Antibody [ARC58154] (A309488) at 1:5,000 dilution. SH-SY5Y cells were treated by Okadaic Acid (100 nM) at 37°C for 1 hour and Calyculin A (100nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-Tau (phospho Ser404) Antibody [ARC58154] (A309488) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Tau (phospho Ser404) Antibody [ARC58154] (A309488)
Western blot analysis of various lysates, using Anti-Tau (phospho Ser404) Antibody [ARC58154] (A309488) at 1:5,000 dilution. Rat brain cells were treated by λ-PP mixed solution (1ul) at 30°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
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