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Anti-Tartrate Resistant Acid Phosphatase Antibody (A14037)

Western Blot - Anti-Tartrate Resistant Acid Phosphatase Antibody (A14037) - Antibodies.com Western Blot - Anti-Tartrate Resistant Acid Phosphatase Antibody (A14037) - Antibodies.com Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase Antibody (A14037) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Tartrate Resistant Acid Phosphatase for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-Tartrate Resistant Acid Phosphatase Antibody
Description
Rabbit polyclonal antibody to Tartrate Resistant Acid Phosphatase.
Applications
WB, ICC/IF
Dilutions
WB: 1:100-1:500, ICC/IF: 1:50-1:200
Reactivity
Human, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 76-325 of human ACP5 (NP_001602.1).
Sequence
TGVQDINDKRFQETFEDVFSDRSLRKVPWYVLAGNHDHLGNVSAQIAYSKISKRWNFPSPFYRLHFKIPQTNVSVAIFMLDTVTLCGNSDDFLSQQPERPRDVKLARTQLSWLKKQLAAAREDYVLVAGHYPVWSIAEHGPTHCLVKQLRPLLATYGVTAYLCGHDHNLQYLQDENGVGYVLSGAGNFMDPSKRHQRKVPNGYLRFHYGTEDSLGGFAYVEISSKEMTVTYIEASGKSLFKTRLPRRARP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
37 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Acid phosphatase 5 tartrate resistant, Acid phosphatase 5, tartrate resistant, ACP5, EC 3.1.3.2, MGC117378, PPA5_HUMAN, serum band 5 tartrate-resistant acid phosphatase, SPENCDI, T5ap, Tartrate resistant acid ATPase, Tartrate resistant acid phosphatase type 5, Tartrate resistant acid phosphatase type 5 precursor, Tartrate-resistant acid ATPase, Tartrate-resistant acid phosphatase type 5, TR AP, TR-AP, TRACP 5, TRAP, TrATPase, Type 5 acid phosphatase
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Tartrate Resistant Acid Phosphatase Antibody (A14037) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Tartrate Resistant Acid Phosphatase Antibody (A14037)
Western blot analysis of extracts of various cell lines, using Anti-Tartrate Resistant Acid Phosphatase Antibody (A14037) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-Tartrate Resistant Acid Phosphatase Antibody (A14037) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Tartrate Resistant Acid Phosphatase Antibody (A14037)
Western blot analysis of extracts of Rat liver, using Anti-Tartrate Resistant Acid Phosphatase Antibody (A14037) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase Antibody (A14037) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase Antibody (A14037)
Immunofluorescence analysis of human lung cancer using Anti-Tartrate Resistant Acid Phosphatase Antibody (A14037) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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