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Anti-Talin 2 Antibody [ARC2335] (A309077)

Western Blot - Anti-Talin 2 Antibody [ARC2335] (A309077) - Antibodies.com Immunohistochemistry - Anti-Talin 2 Antibody [ARC2335] (A309077) - Antibodies.com Immunofluorescence - Anti-Talin 2 Antibody [ARC2335] (A309077) - Antibodies.com Immunofluorescence - Anti-Talin 2 Antibody [ARC2335] (A309077) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2335] antibody to Talin 2 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Talin 2 Antibody [ARC2335]
Description
Rabbit monoclonal [ARC2335] antibody to Talin 2.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Talin 2 (Q9Y4G6).
Sequence
VLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESVSPKKSTILQQQFNRTGKAEHGSV
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2335
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
272 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DKFZp451B1011, DKFZp686I0976, DKFZp686K0979, ILWEQ, KIAA0320, Talin-2, talin2, TLN 2, TLN2, TLN2_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Talin 2 Antibody [ARC2335] (A309077) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Talin 2 Antibody [ARC2335] (A309077)
Western blot analysis of extracts of various cell lines, using Anti-Talin 2 Antibody [ARC2335] (A309077) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-Talin 2 Antibody [ARC2335] (A309077) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Talin 2 Antibody [ARC2335] (A309077)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Talin 2 Antibody [ARC2335] (A309077) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Talin 2 Antibody [ARC2335] (A309077) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Talin 2 Antibody [ARC2335] (A309077)
Immunofluorescence analysis of rat brain cells using Anti-Talin 2 Antibody [ARC2335] (A309077) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Talin 2 Antibody [ARC2335] (A309077) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-Talin 2 Antibody [ARC2335] (A309077)
Immunofluorescence analysis of mouse brain cells using Anti-Talin 2 Antibody [ARC2335] (A309077) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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