Anti-TAF15 Antibody (A10428)

Name: Anti-TAF15 Antibody
Brand: Antibodies.com
SKU: A10428
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to TAF15 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-TAF15 Antibody

Description

Rabbit polyclonal antibody to TAF15.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TAF15 (NP_631961.1).

Sequence

MSDSGSYGQSGGEQQSYSTYGNPGSQGYGQASQSYSGYGQTTDSSYGQNYSGYSSYGQSQSGYSQSYGGYENQKQSSYSQQPYNNQGQQQNMESSGSQGGRAPSYDQPDYGQQDSYDQQSGYDQHQGSYDEQSNYDQQHDSYSQNQQSYHSQRENYSHHTQDDRRDVSRYGEDNRGYGGSQGGGRGRGGYDKDGRGPMTGSSGGDRGGFKNFGGHRDYGPRTDADSESDNSDNNTIFVQGLGEGVSTDQVGEFFKQIGIIKTNKKTGKPMINLYTDKDTG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

68 kDa TATA-binding protein-associated factor, Npl3, RBP56, RBP56_HUMAN, RNA binding protein 56, RNA-binding protein 56, TAF, TAF(II)68, TAF15 RNA polymerase II TATA box binding protein (TBP) associated factor 68kDa, TAF2N, TAFII68, TATA-binding protein-associated factor 2N

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-TAF15 Antibody (A10428)

Western blot analysis of extracts of various cell lines, using Anti-TAF15 Antibody (A10428) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 5s.

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Figure 2: Immunohistochemistry - Anti-TAF15 Antibody (A10428)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-TAF15 Antibody (A10428) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Western Blot - Anti-TAF15 Antibody (A10428)

Immunoprecipitation analysis of 200µg extracts of SKOV3 cells using 3µg of Anti-TAF15 Antibody (A10428). This Western blot was performed on the immunoprecipitate using Anti-TAF15 Antibody (A10428) at a dilution of 1:1000.

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