Anti-TAF15 Antibody [4D71] (A85450)

HeLa cell cultures stained with Anti-TAF15 Antibody (green) and Anti-Vimentin Antibody (A85421 | red). Anti-TAF15 Antibody reveals a granular nuclear localization typical of many RNA associated proteins. Blue is a DNA stain.

Immunofluorescent analysis of HeLa cells stained with Anti-TAF15 Antibody [4D71 ] (A85450), at a dilution of 1:500 in red, and co-stained with Anti-GAPDH Antibody (A85377), at a dilution of 1:1,000 in green. Blue is Hoechst staining of nuclear DNA. The Anti-TAF15 Antibody [4D71 ] (A85450) reveals TAF15 protein expressed exclusively in the nuclei of the cells, while Anti-GAPDH Antibody (A85377) produces strong cytoplasmic staining.

Western blot analysis of cytosol or nuclear enriched fractions of cell line lysates using Anti-TAF15 Antibody [4D71] (A85450), at a dilution of 1:1,000, in green. The lanes contain samples of: [Lane 1] Protein standards, in red, [Lane 2] NIH-3T3 cytosol, [Lane 3] NIH-3T3 nuclear fraction, [Lane 4] HeLa cytosol, and [Lane 5] HeLa nuclear fraction. Double band at 56kDa and 68kDa detected predominantly in the nuclear enriched fraction corresponds to the TAF15 protein.

Blot of 20 µg HeLa cell lysate (Lane 1) and 20 µg HEK293 cell lysate (Lane 2) was probed with Anti-TAF15 Antibody, at a dilution of 1: 1,000). The Anti-TAF15 Antibody binds strongly and cleanly to a band at about 68 kDa coresponding to TAF15.