TAA06 Biosimilar - Anti-CD276 Antibody - BSA and Azide free
Description
Recombinant humanized monoclonal antibody to CD276.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Humanized
Clonality
Monoclonal
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
B7H3 protein is expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with 1µg/ml TAA06 Biosimilar - Anti-CD276 Antibody - BSA and Azide free (A324692) on Expi293 cells transfected with human B7-H3 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).
ELISA plates were pre-coated with Recombinant Human CD276 Protein (Fc Chimera 6xHis Tag) (A318396) at 2 µg/ml (100 µl/well) which can bind TAA06 Biosimilar - Anti-CD276 Antibody - BSA and Azide free (A324692) in a linear range of 0.64-16 ng/ml.
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