Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag)
Description
Full-length transmembrane human Claudin18.2 protein (EGFP tag) expressed on the cell surface of Virus like particles.
Applications
ELISA, FACS
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human Claudin18.2 protein has a MW of 54.2 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Figure 1: Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag) (A318480)
ELISA plates were pre-coated with 0.5 µg/per well Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag) (A318480). Serial diluted Anti-Claudin18.2 Chimeric Antibody [Zolbetuximab Biosimilar] - Azide free (A318887) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-Claudin18.2 Chimeric Antibody [Zolbetuximab Biosimilar] - Azide free (A318887) binding with CLDN18.2-EGFP-VLP is 877.5 µg/ml.
Figure 2: Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag) (A318480)
FACS analysis of Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag) (A318480): A. Negative Control 1: Expi293 cells were stained with Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag) (A318480). B. Negative Control 2: Anti-GPRC5D-CAR-Expi293 cells (an irrelevant CAR) were stained with Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag) (A318480). C. Positive Control: Anti-CLDN18.2-CAR-Expi293 cells were stained with biotin labeled Protein L, followed by Streptavidin-APC Antibody. D. Anti-CLDN18.2-CAR-Expi293 cells were stained with Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag) (A318480).
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