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Synthetic Nanodisc Human TRPV1 Protein (A317302)

ELISA - Synthetic Nanodisc Human TRPV1 Protein (A317302) - Antibodies.com Western Blot - Synthetic Nanodisc Human TRPV1 Protein (A317302) - Antibodies.com
$1,300
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Name
Synthetic Nanodisc Human TRPV1 Protein
Description
Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human TRPV1 protein to be purified and analysed in vitro.
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human TRPV1 protein has a MW of 94.8 kDa.
Conjugate

Unconjugated

Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
Capsaicin receptor, DKFZp434K0220, osm 9 like TRP channel 1, Osm-9-like TRP channel 1, OTRPC1, Transient receptor potential cation channel subfamily V member 1, TRPV 1, TRPV1_HUMAN, Vanilloid receptor 1, Vanilloid receptor subtype 1, VR 1, VR1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA - Synthetic Nanodisc Human TRPV1 Protein (A317302) - Antibodies.comEnlarge Image
Figure 1: Synthetic Nanodisc Human TRPV1 Protein (A317302)
ELISA plates were pre-coated with Synthetic Nanodisc Human TRPV1 Protein (A317302) at 0.2µg/per well. Serial diluted Anti-Flag Monoclonal Antibody solutions were added, washed, and incubated with a secondary antibody before reading the ELISA. From the above data, the EC50 for Anti-Flag Monoclonal Antibody binding with Synthetic Nanodisc Human TRPV1 Protein (A317302) is 41.79ng/ml.
Western Blot - Synthetic Nanodisc Human TRPV1 Protein (A317302) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Synthetic Nanodisc Human TRPV1 Protein (A317302)
Western Blot analysis of Synthetic Nanodisc Human TRPV1 Protein (A317302) with Anti-Flag Monoclonal Antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution.
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