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Synthetic Nanodisc Human TLR2 Protein (A318416)

ELISA - Synthetic Nanodisc Human TLR2 Protein (A318416) - Antibodies.com SDS-PAGE - Synthetic Nanodisc Human TLR2 Protein (A318416) - Antibodies.com Synthetic Nanodisc Human TLR2 Protein (A318416) - Antibodies.com
$1,300
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$40 to United States
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7-8 Business Days

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Name
Synthetic Nanodisc Human TLR2 Protein
Description
Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human TLR2 protein to be purified and analysed in vitro.
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human TLR2 protein has a MW of 89.8 kDa.
Conjugate

Unconjugated

Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
CD282, CD282 antigen, TIL 4, TIL4, TLR 2, TLR2_HUMAN, Toll like receptor 2, Toll like receptor 2 precursor, Toll-like receptor 2, Toll/interleukin 1 receptor like 4, Toll/interleukin 1 receptor like protein 4, Toll/interleukin receptor like protein 4, Toll/interleukin-1 receptor-like protein 4
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA - Synthetic Nanodisc Human TLR2 Protein (A318416) - Antibodies.comEnlarge Image
Figure 1: Synthetic Nanodisc Human TLR2 Protein (A318416)
ELISA plates were pre-coated with Synthetic Nanodisc Human TLR2 Protein (A318416) (0.2 µg/well). Serial diluted Anti-Flag Tag Antibody solutions were added, washed, and incubated with secondary antibody before ELISA reading. From this data, the EC50 for Anti-Flag Tag Antibody binding with TLR2-Nanodisc is 2.685 µg/ml.
SDS-PAGE - Synthetic Nanodisc Human TLR2 Protein (A318416) - Antibodies.comEnlarge Image
Figure 2: SDS-PAGE - Synthetic Nanodisc Human TLR2 Protein (A318416)
Synthetic Nanodisc Human TLR2 Protein (A318416) on SDS-PAGE under reducing conditions.
Synthetic Nanodisc Human TLR2 Protein (A318416) - Antibodies.comEnlarge Image
Figure 3: Synthetic Nanodisc Human TLR2 Protein (A318416)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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