Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human Claudin18.2 protein to be purified and analysed in vitro.
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human Claudin18.2 protein has a MW of 27.5 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Figure 1: Synthetic Nanodisc Human Claudin18.2 Protein (A318483)
ELISA plates were added with Synthetic Nanodisc Human Claudin18.2 Protein (A318483) (0.2 µg/per well) on an Anti-Flag Tag Antibody pre-coated (0.2 µg/per well) plate. Serial diluted Anti-Claudin18.2 Chimeric Antibody [Zolbetuximab Biosimilar] - Azide free (A318887) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-Claudin18.2 Chimeric Antibody [Zolbetuximab Biosimilar] - Azide free (A318887) binding with Synthetic Nanodisc Human Claudin18.2 Protein (A318483) is 593.6 µg/ml.
Figure 3: Synthetic Nanodisc Human Claudin18.2 Protein (A318483)
Synthetic Nanodisc Human Claudin18.2 Protein (A318483) can bind Anti-Claudin18.2 Chimeric Antibody [Zolbetuximab Biosimilar] - Azide free (A318887) with an affinity constant of 1.619 nM as determined in a SPR assay.
Figure 4: Synthetic Nanodisc Human Claudin18.2 Protein (A318483)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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