Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human CD37 protein to be purified and analysed in vitro.
Applications
Western Blot, ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human CD37 protein has a MW of 31.7 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 1: Synthetic Nanodisc Human CD37 Protein (A318458)
ELISA plates were pre-coated with Synthetic Nanodisc Human CD37 Protein (A318458) (0.2 µg/well). Serial diluted Anti-CD37 Chimeric Antibody [Naratuximab Biosimilar] - Azide free (A318910) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From this data, the EC50 for Anti-CD37 Monoclonal Antibody binding with CD37-Nanodisc is 23.06 µg/ml.
Figure 3: Western Blot - Synthetic Nanodisc Human CD37 Protein (A318458)
Western blot analysis of Synthetic Nanodisc Human CD37 Protein (A318458) with Anti-CD37 Chimeric Antibody [Naratuximab Biosimilar] - Azide free (A318910), followed by Goat Anti-Human IgG Antibody (HRP) at 1:5,000 dilution.
Figure 4: Synthetic Nanodisc Human CD37 Protein (A318458)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
Publishing research using Synthetic Nanodisc Human CD37 Protein (A318458)? Please let us know so that we can list the citation on this page.
Alternative products to Synthetic Nanodisc Human CD37 Protein (A318458)
